Chris Browne, UR - Tentative BacteriaMembrane Filter, glass,

      Alright, per Dr. Woodall, let’s discuss my methods more closely, and when we have set up the research itself. So were going to be taking a nearly identical approach to the filtration system, just like total suspended solids. It all starts with canal street, New Smyrna Beach. Scheduling with the college's main lab, and Dr. Woodall puts the majority of my research in the beginning of April.

Glass Filter, 0.45 microns

     

     Once I have collected the sample, I would follow the USEPA Membrane Filtration Method, as outlined and with the appropriate professor’s assistance. A minimum of two samples will be used to compare conditions for bacteria. My paper will have some background research of other studies with similar experiments.

Filter Assembly

1         .       Bring entire set up to Dr. Sandra M. Horikami for Autoclave use,                   ahead of sample and keep isolated until it has cooled and I have                     samples prepared.

2         .       Set up Petri-dish and broth prepared for filtered sample

3         .       Membrane assembly

4         .       Using the vacuum, and appropriate sample size, filter through,                       including washing sides of  membrane assembly with distilled water

5         .       Using the filter itself, carefully apply the filter to the petri dish, and               incubate at 44.5®C for 26 hours.

6         .       After that, it’s time for the microscope, identification, and/or counting           the bacteria that has been cultured for research.

          

Prepared / Cultured Petri Dish

 ~CMB