IMES

IMES

Friday, March 30, 2018

Katie UR: It's Called Fishing, Not Catching.

Since last Friday my whole family has been nothing but helpful in trying to help me catch sharks to get swabs of their mouths. These last three days we have done nothing but gone out fishing for hours on end just to be a little disappointing that we didn't catch anything. But i waned to break down each of the spots we went to attempt at catching a shark!

Site 1
Location: Flagler Beach Park Under State Road Bridge
Date:03/26/2018
Time in: 9:36pm
Time Out: 12:56am
Compass:29.28'42 N 81.8'W
Temp:74 Degrees
Feet From Bridge:63 feet
Condition: Cool out and windy.
Poles out:9 poles out with frozen Mullet on the hooks
Details: One pole got a lot of action and the bait was tore up. We watched the poles being messed with but nothing really bit onto the bait fully


Site 2
Location: Flagler Beach Park Under State Road Bridge
Date: 03/27/2018
Time In: 3:05pm
Time Out: 6:13 pm
Compass: 29.28'39N 81.8'W
Temp: 71 Degrees
Feet From Bridge:64 feet
Condition: Beautiful day out
Poles out:9 poles out again with mullet on the hooks for bait
Details: No action at for this trip. We caught more snapper then we did sharks !

                                Site 3
Ponce Inlet South Jetty
Location:Ponce Inlet Jetty South Side
Date:03/28/2018
Time In:3:30pm
Time Out: 7:01pm
Compass:29.4'42 N 80.5513W
Temp:76 degrees
Elevation:-30 feet
Condition: Very Windy but sunny and cool out
Poles out: We had 3 poles out and had fresh mullet on the hooks as bait.
Details: Each pole was hit instantly when put .We replaced bait a couple times and we are planning on going back here more often !


My Little Brother Dean Out on The Jetty






It was a lot of fun to get my whole family into something that is so important to me. My little brothers had the most fun out of everything. We are going to continue to go out every chance we take and for as long as we can. Like It says in the title, It's called fishing, not catching!







Sheep's Blood Agar Plates




Transport CultureSwab 
Lastly, Dr. Horikami has ordered me swabs that are going to be a huge help for my project. These Transport CultureSwabs are the same ones that the previous studies mentioned used. She also got me TSA medium petri dishes for me to use to spread the swab onto. This will allow us to classify the bacteria that is in the sharks mouths.

Tuesday, March 27, 2018

Jenna (ur)- Sulfur grabs attention?

Doing research on seabirds and plastics, i have found that most of the birds near our coast consist of brown pelicans and laughing guls which stay on our coast year round. Other birds include northern gannets, piping plovers and white pelicans. These are migratory birds that are usually here around spring and early summer. Birds generally mistake plastics for food because it looks like the birds natural prey. Marine plastic debris also put off an odor that the birds associate with food. Certain tube-nose sea birds are attracted to dimethyl sulfide, which is a natural scented sulfur compound. Plastics that have been in the ocean pick up that scent from algae.
Laughing gulls in New Smyrna Beach
Brown Pelican

Cynthia UR Proposal


A study in Anthropogenic Effects on Florida Estuary Plankton in the South Atlantic Region
By Cynthia Sharp IMES Program OCE 2013

     q  Phytoplankton transform carbon dioxide during respiration releasing oxygen in the process.
 Half of earth’s oxygen is produced by photosynthesis by phytoplankton; our ocean marine plants estimated production as a whole is 70 to 80 percent.  Though at night this process uses some oxygen it is firmly believed that this process produces more oxygen then it uses while capturing large amounts of carbon.  By Biosequestration, the algae is now being considered for use as an organic biofuel.

     q Anthropogenic Activities effect nutrient and bacterial balances. 

Much is acknowledged as to effects of nutrient loading.  It produces algae blooms in rivers, lakes, estuaries and our shorelines.  The initial blooms and eutrophication that follow rob the life giving oxygen reserves, resulting in large scale kills of fish and other sea creatures that eventually wash ashore in great numbers. 

q  The potential change to marine plankton biodiversity, would intern impact all other organisms dependent on them.
The imbalance then too is created amongst creatures that once fed, or continue to feed on these coastal phytoplankton and those effected by the increase in bacteria both anaerobic on the seafloor, and by aerobic free swimmers.
This week I will include the species we expect, based on what is known to be in the estuaries.
Can we find differences at the microscopic plankton species level between the anthropogenic and natural pristine areas?

  • Collect samples from both types of sites
  • Identify microscopic organisms that occupy these samples                    .
  • Document Classifications and variety of plankton                                              .
  • Look for patterns between location types and species.
Observations for this study will be done at four sites




a)Halifax River -Ormond Beach
Haw Creek Preserve State Park
b) Halifax River -Daytona Beach
Near Mason Bridge
c) Indian River North -New Smyrna Beach
Near Causeway Bridge
d) Indian River -New Smyrna Beach

Canaveral National Seashore



The study will consist of four samples in four separate tanks at 5.7 Liters, or about 1.5 gal.

A soft source of current can be delivered by a pump. 


Products like “Top Fin” can be used as evaporation occurs.


A timer along with a sunlamp will provide source to continue the processes of photosynthesis. 

The samples will stay at room temperature other then what heat absorbed by sunlamp. (approximately 12hrs and 10 mins. Each day) 
  • To identify microscopic organisms

Record individuals to their locations according to sample
Document differences if they exist
Use bar graphs to illustrate and reveal patterns
Possibly use plots to correlate similar results from samples. 
Monitor to discover and further research

The Changes that take place
What diversity deviations can we distinguish
Distance or region effected by these changes
Document over time to recognize spread
Synergistic variation due to Season and environment

Are the processes altered or increased


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Chad Macfie, Curator of live exhibits 
Marine Science Center  Daytona Beach, Florida
Pamela Hopkins, PhD Research Associate,
Florida Oceanographic Society  Stuart, Florida
Kirby, Richard. R. (2011) Ocean Drifters a secret world beneath the waves.  
Buffalo, NY: Firefly Books (U.S.) Inc.      
Bermuda Institute of Ocean Sciences /BiosScope
National Centers for Coastal Ocean Science (NCCOS) https://products.coastalscience.noaa.gov/pmn/gallery.aspx
Jennifer Chu / Massachusetts Institute of technology (MIT) News July 20, 2015




On April 2, 2018 at 5:20 PM I got a question from
Jessy 102 section. What plankton species are known to live in our area?
I don't know much about this but sounds really cool!
Crustaceans ReaserchGat

Hi Jessy,
So, plankton, sizes up to 5mm down to only a few micrometers come in many forms; three types are Phytoplankton, Holoplankton and Miroplankton. Phytoplankton perform photosynthesis and unless I discover colonies, (diatoms and others form distinct groupings) these will often be too small to observe with my equipment. However Holoplankton spend their entire lives as plankton, most feeding on phytoplankton and the others Miroplankton spend only part of their lives as plankton on their way to becoming adult creatures of everything imaginable. Cnidarians and Ctenophores, phylum’s that include true jelly fish, sea anemones and pens, many with multiple stages; Crustaceans such as Copepods that have six stages and nine orders most of those parasitic; Amphipods and Euphausid (krill) I am still working on.  Mollusca larvae too could be present. It’s extensive because of Florida and my estuary choice of locations.


Florida’s unique position south along the Atlantic coast and upwards of the Caribbean warm water temperatures place it in prime upwelling zones bring nutrients to coastlines. As the Sea warms through the seasons these zones increase up north and the summer months in the tropics will actually see a decrease in plankton once nutrients are used. Florida resides alongside the Gulf Stream and nutrient rich all year long and 2018 is a La Nina year increasing the plankton activity. Thanks for asking, it will be an interesting exploration.

Lindsey, R. & Scott, M. NASA. (2010). What are phytoplankton? Earth Observatory. Retrieved from

https://earthobservatory.nasa.gov/Features/Phytoplankton/page1.php


Miss Veronica wrote....
Hello Cynthia! 
I'm Veronika Goncharova from OCE1001 section 301. I had a question about something you mentioned in your introduction. You said that "By Biosequestration, the algae is now being considered for use as an organic biofuel." What is biofuel? Can algae be used as an energy source because of how much there is of it? Would it be more useful to use algae from anthropogenic or natural pristine areas? Would this interfere with plankton producing oxygen? Please let me know!! 


04/10/2018
Hi Veronica,
Unlike fossil fuels, biofuels, (this includes ethanol’s derived from starch and sugars) are derived from living organisms. Algae’s tight community and ability to capture carbon (autotrophic producers) make it a harvestable carbon resource for consideration. 

According to the office of energy efficiency and renewable energy some algae produce oils they hope to harvest in the future.


As to the last part of your question I’m sure it’s a question that still needs to be answered. We use algae now creating an abundance treating waste waters, but have we discovered them all? Much of the microscopic world is yet to be revealed and cyanobacteria that are considered phytoplankton are assuming a place in the future as well. I will not be discovering this answer in my study but definitely worthy of consideration.



Katie UR: Shark On!

My project is on Bacteria that is found in a sharks mouth. Right now I am just comparing the bacteria to each other and classifying them. After this semester hopefully I can get more connections and help to figure out which of the bacteria is drug-resistant. But let me not jump the gun. 

My proposal for you is Bacteria: The Untold story From A Sharks Mouth. Like I mentioned before this is a project that is hopefully going to grow with me as I go through school.

Unger using his Swabbing Device to get a Sample
What is known: The known is that a previous study was done in August of 2014. The capturing of the sharks took place in February and April of 2013 while the testing of the bacteria study was done the following the January. The whole study didn't get published until August of 2014. Nathan R Unger and a group of scientists and fishermen gathered together to work on the bacterial infections that are caused from bacteria transferred from sharks to humans when there are shark bite attacks. They based the study in Palm Beach along the Florida Coast. They caught 19 Blacktip Sharks and swabbed their mouths with Transport Tubes and took them back to the lab to e spread on several agar plates. What they found of drug-resistant bacteria in Blacktip Sharks alone is crazy! There was a 12% resistant bacteria found in Blacktips mouths. 
Now for the known about the location I am using to catch the sharks at. Ponce Inlet has had an active year last year with shark attacks. In a 4 month span last year, through June to October there were 4 separate shark attacks off of Ponce Inlet. Just two days ago there was a Great White Spotted off of Turtle Mound in Ponce Inlet! A Great White would be the best swab to get !

My Scientific Question: Is Bacteria only common in certain species of sharks or does it vary from shark to shark? If it does vary,is it more extreme in certain sharks then in others?

My Materials and Methods:
Field Materials: Swabbing device, hooks,grade line, wire line, bite down stick, and a Boat obviously!

Microbiological Materials: Transport CultureSwab, petri dishes with the 5 mediums in them, santi-bags, gloves for transport, and an Ice Chest for transport back to the lab.

Methods: There will be 3 methods used for this project that will be hopefully repeated with each shark exactly the same, but we all know that wont happen!

Pen Fierce 8000 Rod With 50 Pound braided line
 The first method will be catching the shark. Doing this I will be using a Pen fierce 8000 Rod with 50 pound braided line and a 6 pound weight and a 16"in Mustad circle hook. 
16" Mustad Circle Hook
The bait will either be frozen mullet caught at High Bridge in Ormond and bait we cast net at Ponce Inlet. We will cut the mullet down its belly and place the hook up through it and around the spine so if there is a hit the shark wont be able to just yank the whole bait off, it will be struggle. 

Mullet being cut down the Belly


The second method I will be using will be involving getting the samples of the mouth. Once the shark is caught we will have wet towels to place over their eyes, Thank you to Josh for telling me that fun fact, this will keep them calmer then what they are now. One person will place the bite down bar in the sharks mouth so no one is hurt in trying to get the swab and then also so we can place a hose in the sharks mouth to run saltwater trough their gills. I have about 3 minutes to safely get the swab and release the shark. There will be another person measuring the shark and identifying what shark is it and hopefully the sex. Another person will be focusing on taking just the pictures of everything so everything can handled correctly. The two people who are in charge of the test tubes will both be wearing gloves when the swabbing is taking place. One will hold the tube the other will swab the mouth and place it in the tube being held by the other person. Once the swab is in the tube the person holding the tube will place the lid of the Transport tube back on and place it in the ice chest for transport back to the lab. Back to the shark though, the hose and towel will be removed and the shark will be correctly placed Back into the water and accumulated properly before released.

The third and final method will be the microbiological method. So this method is not as finished as the other two because I have been working with Dr. Horikami and she is a very busy lad but she has been nothing but helpfully. She is supplying me with peteri dishes with Sheeps blood, TSA, MaConkey and two others that will have the bacteria spread on them and we will incubate them and watch them grow! Then identify the bacteria that is grown on it. Once this method is properly done then I will inform you guys on this!

Data I can Use: For this project I can either use a table to show my data, a clustered bar graph to show the species of shark and the bacteria they hold or a single pie graph for each shark caught to show the percentage of each bacteria in the sharks mouths.


So let me know what you guys think of the ideas and please share yours!

Rachel UR, Roseate Spoonbill Migratory Range


 This past Friday I had the opportunity to present my research proposal on the Roseate Spoonbill and whether this bird species is migrating further north.


 The photo above is one I had taken this past January, in the center you can see a Roseate Spoonbill taking off mid-flight. Historically, the Roseate Spoonbill is a rare site to see north of Titusville, FL in the winter months (Dumas). Being that I had previous knowledge on this particular birds migratory range, I was pretty surprised to see them flying around the Ormond beach area in the middle of the winter.

 One method of collecting data for this type of research will be the utilization of bird surveys from the Nation Audubon Society. A bird survey is a record of how many times a certain bird was sited in a specific location. The majority of my research will be pulled out of the Audubon's Christmas bird surveys. This survey has been conducted for 100 years on every December 14th - January 5th. Since I can not find published research on this bird's migratory range after the year 1996, I will be pulling surveys from the years 1996-2017,  and looking to see if there has been more sightings in northern locations in the past ten years than previous years.

                        


    One thing I would like to include in my research is a graph of the average temperature in winter months for the years 1996-2017, to see if there is any correlation between rising temperatures and the Roseate Spoonbill's dispersal north.



Dumas, J.V.2000. Roseate Spoonbill (Platalea ajaja), version 2.0. In The Birds of North America (A.F. Poole and F.B. Gill, Editors). Cornell Lab of Ornithology, Ithaca, NY, USA, Retrieved from https://doi.org./10.2173/bna.490



Niki UR How Safe is Our Water?

My project is about the movement of water nutrients through the Ponce Inlet water system from the Halifax River and The Indian River North. 
What is known: 
Total Nitrogen and Phosphorous are essential to healthy plant and animal growth, can cause algae blooms in overabundance 
Dissolved oxygen: essential for plant and animal growth, not enough causes eutrophication of habitat 
Total Suspended Solids: can include silt, decaying matter, or industrial waste
Salinity: Hypersaline: 38ppt
              Hyposaline: <30 ppt




   The Indian River North is the uppermost section of the Indian River Lagoon, spreading from the Ponce Inlet to Oak Hill. I will be focusing on the northernmost part, closest to the Inlet itself. 

The Halifax River runs from the Ponce Inlet to Ormond Beach, and is 25 miles long, again I will be focused on the  area closest to the Inlet, or the southernmost section. 

My scientific question may still need some work, but I want to experiment on and determine the water quality of the Halifax River, Indian River North and Ponce Inlet and see if these conditions are up to EPA standards.














Materials and Methods:
 YSI 85 Oxygen Conductivity Salinity Temperature (and pH)
HACH Calorimiter DR 890 (total nitrogen and phosphorus) 
HACH 2100 N TURBIDIMITER
HACH 2100 N Turbidimeter

HACH Calorimeter DR 890








 





YSI 85 Oxygen Conductivity Salinity Temperature

Halifax River: one sample at outgoing tide
Indian River North: one sample at outgoing tide
Ponce Inlet: one sample at outgoing tide, one sample at incoming tide


My data will be represented by a cluster bar graph, with all of the information from each site in its own cluster, with pH, salinity, DO, TN and TP to see an accurate comparison between waterbodies, another ID can be created for EPA standards. 


Perlman, Howard, and USGS. “Nitrogen and Water.” Nitrogen and Water: USGS Water Science School, 17 Jan. 17AD, water.usgs.gov/edu/nitrogen.html.
USF Water Institute, School of Geosciences, A, and A University of South Florida. “Tampa Bay Water Atlas.” Welcome to the Water Atlas, 2018, www.tampabay.wateratlas.usf.edu/.

 

Karen (UR) Got Weed ?

Have I got your attention now?  Thought so.....  :-)

My Research Project is on Duckweed, Lemna minor, and it's ability to filter excess nutrients (Nitrate and Phosphate) from fresh water. This tiny and I mean tiny plant can do big things.
 

This is what is known: For centuries, in Asia, small scale farmers have used this plant to filter out excess nutrients from their "sludge" ponds.  A sludge pond is where waste is kept, it can be animal or plant waste, and turned into fertilizer. Duckweed also purifies the water so that it can be reclaimed for watering crops and livestock. Because Duckweed can double it's mass in 48 hours it is also used as a high protein food source for humans, animals and fish, it has become a multi purpose plant.  Yes, the plant is rinsed and dried before being used.  Recently the University of North Carolina has been conducting research on this subject.  Many other countries have been using this plant for the above purpose as well.  It has been particularly useful in  the Middle East and Africa.

What is not known:  Is Lemna minor a possible answer for the bioremediation of excess nutrients from fresh water?

My scientific question:  Is there a concentration level that is lethal to the Duckweed?


I will be using these materials:

Distilled Water,  LaMotte Nitrate/Phosphate test kit #3119, 4 1/2 inch Culture Dishes, Miracle Gro 8-7-6 Liquid Fertilizer, Biotronette Mark III Environmental Chamber

Methods:

Using the above materials, 4 culture dishes will be prepared with 150 ml of

DI water and liquid fertilizer, 1 as a control, 3 with duckweed.  
Culture dishes will be placed under plant light in the chamber with 12 hours

of daylight.
Every 48 hours the liquid will be checked for evaporation/transpiration loss

and adjusted accordingly using the fertilizer/ DI water mixture. 
The DI water/fertilizer mixture in the dishes will be tested for Nitrate levels.


References:  
Ansari, A. A., & Khan, F. A. (2008). Remediation of eutrophic water using Lemna minor in a controlled environment. African Journal Of Aquatic Science, 33(3), 275-278. doi:10.2989/AJAS.2008.33.3.11.623
Burkholder, J., Libra, B., Weyer, P., Heathcote, S., Kolpin, D., Thorne, P. S., & Wichman, M. (2007). Impacts of Waste from Concentrated Animal Feeding Operations on Water Quality. Environmental Health Perspectives, 115(2), 308–312. http://doi.org/10.1289/ehp.8839
“Wildflower Preserve Water Quality Initiatives.” Lemon Bay Conservancy, lemonbayconservancy.org/wildflower-preserve/wildflower-preserve-water-quality-initiatives/.