Chris Browne, UR - Location Change, Bacteria

Petri Dish Example

     Canal Street is off limits. Looks like I’m teaming up with another local body of water with Emily Reyes. Suggested by Dr. Woodall, I will be doing the same IRP, just at Emily’s location. We will be testing for excessive depositional nutrients and coliform bacteria, including the big/little E. coli.

Tuscawilla Park, (Pic. ref. Emily R.)

     

     Question: Is E.coli and/or other bacteria found in the runoff area of Tuscawilla Park before and after rainfall, 

and if so just how much and what types are there?

Filtration Set-up

     

Method

Materials: -Sampling container

 (2 tests, 1 control)

-Autoclave

-Membrane Filter Unit:

  Funnel manifold, flask, glass

  Filter (0.45) microns, vacuum

-forceps, petri dish

-BGB broth

-Incubator

-Microscope

     

     

                                              Methods:  1. Sample size should be 20mL min, and the same size

          ~CMB                                                For each sample.

                                                               2. Autoclave entire filtration manifold.

                                                                   24 hours plus cool down, wrapped

                                                           3. Filter method, and set up filter on petri dish, incubate

                                                                 24 hours isolated

                                                        4. Count colonies of bacteria, using formula, report results.

      Equation for results: Coliform colonies per 100ml = Coliform colonies counted  X  100

                                                                                              mL of sample filtered