Coliforms are classified as. E. coli is a fecal bacteria
that is found naturally in the environment, but more specifically around waste
water, sewage, and places where people live. My paper will research how
bacteria can affect the surrounding marine and waterway environments. My
research will include water samples from Canal Street, new Smyrna beach. |
| Bacteria is everywhere. |
I’ll be
using the USEPA Membrane Filtration Method, 8074 with help from the Science
department head, Dr. Horikomi. To paraphrase the process of research, I’ll
break it down into three pieces, the filtration, Incubation, and examination. Filtration
will start with using an autoclave to completely sterilize the funnel and
filter assembly. Equipment used will be a membrane filter, funnel, manifold,
Incubator, forceps’, BGB Broth, Agar plates, and filter set up with a 0.45
micron pore size to catch the desired bacteria. A prepped sample will be run through
the filter. A vacuum will be used, for the filtration process.
 |
| Filter Assembly |
I will incubate the cultures on a Petri dish,
making sure there is no air where the filter meets the cultures broth (food
source), for 24 hours. Followed by a microscope examination, counting the
stained blue colonies of bacteria in the given dish. Recording results, I’ll
make an interpretive statement of findings, based on the accepted forms of
datum reporting. I believe by using this filtration process I will be able to
identify and count how numerous bacteria is in the canal street waterway, and summarize
from other research the sources, and how bacteria can be reduced, as to respect
our marine environments.
~CMB
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